Part:BBa_M1659:Design

Composite part for lung cancer biomarker

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal XhoI site found at 143
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 636
  Illegal SapI.rc site found at 531
  Illegal SapI.rc site found at 1164
  

Design Notes

it has a promoter sequence, ribosome binding site, histag, coding region and a terminator

Check for restriction enzyme sites.

Source

source is human genome which has been optimized according to e.coli genome.